强直性脊柱炎检测HLA-B27、ASO、RF、CRP的临床意义

The Clinical Significance of Testing HLA-B27、ASO、RF、CRP in Ankylosing Spondylitis

目的探讨HLA-B27、ASO、RF、CRP检测在强直性脊柱炎(AS)诊断与鉴别诊断中的意义。方法抽取ED-TA(K2)抗凝血2ml,采用磁珠酵素免疫法检测HLA-B27,采用胶乳凝集法检测ASO、RF、CRP。结果 31例正常健康者加A-B27、ASO、RF、CRP检测结果均为阴性。108例疑似AS患者中,HLA-B27阳性率为30.6％(33/108)。33例HLA-1327阳性患者中,ASO阳性率为6.1％(2/33),RF阳性率为3.0％(1/33),CRP阳性率为12.1％(4/33)。结论 HLAA-B27的检测可明确AS诊断,特别是在不典型AS诊断中具有重要的临床价值。ASO、RF检测可用于AS的鉴别诊断。疑似AS患者HLA-B27阳性者中,其活动期的CRP比率较低。

Objective Investigate the significance of testing HLA-B27、ASO、RF、CRP in Ankylosing Spondylitis (AS) diagnosis and differential diagnosis. Method We withdraw EDTA (K2) anticoagulant blood 2ml, adopt IMS ELISE to test HLA-B27, adopt latex agglutina- tion to test ASO、RF、CRP. Results The HLA - B27、ASO、RF、CRP test result of thirty - one normal healthy are all negative. In one hun- dred and eight suspected AS patients, HLA- B27 positive rate is 30.6% (33/108). In thirty-three HLA-B27 positive patients, ASO posi- tive rate is 6.1% (2/33), RF positive rate is 3.0% (1/33), CRP positive rate is 12.1% (4/33). Conclusion The testing of HAL- B27 can diagnose AS, especially has important clinical value in diagnosis untypical AS. The testing of ASO、RF can use in the differential diagnosis of AS. In suspected HLA-B27 positive AS patients, its active stage ratio is lower.