蒺藜皂苷对人肝癌细胞BEL7402生长抑制和诱导凋亡作用的研究

Investigation on inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris on hepatoma cell line BEL-7402

目的 :探讨蒺藜皂苷 (STT)对体外培养的人肝癌细胞BEL 74 0 2的增殖抑制作用及诱导凋亡作用。方法 :应用MTT与SRB法检测蒺藜皂苷对人肝癌细胞BEL 74 0 2的抑制作用 ,采用形态学观察以及流式细胞术 (FCM)检测了STT诱导人肝癌细胞BEL 74 0 2凋亡的效应 ,采用流式细胞术检测BEL 74 0 2细胞Bcl 2蛋白表达。结果 :①STT能够剂量依赖地抑制人肝癌细胞BEL 74 0 2的增殖 ;②细胞显现典型的凋亡形态学改变 ,FCM出现凋亡峰 ;③STT能降低BEL 74 0 2细胞的Bcl 2蛋白表达。结论 :STT在体外能抑制BEL 74 0 2细胞的增殖并能诱导细胞凋亡 ,其诱导凋亡作用的途径之一是下调Bcl 2蛋白表达。

Objective: To investigate the inhibitory and apoptosis-inducing effects of saponins from Tribulus terrestris (STT) on liver cancer cell line BEL-7402. Method: MTT, SRB, Wright staining, acridine orange staining, flow cytometry, and Immunofluorescence microscopy were used to evaluate the effects of STT on BEL-7402 cell line. Result: STT had potent inhibitory effect on BEL-7402 cell line in a concentration-dependent manner. BEL-7402 cells exibited typical morphological alteration of apoptosis when sub-G1 peak could be seen. The expression of Bcl-2 was decreased in STT treated cells as compared with untreated control cells. Conclusion: STT exerts its cytotoxic effect on BEL-7402 cells by inducing apoptosis.