摘要
目的 探讨天然和氧化极低密度脂蛋白 (n VLDL ,ox VLDL)对培养的人脐静脉内皮细胞 (HUVEC)表达血管细胞粘附分子 1(VCAM 1)的影响。方法 将n VLDL和ox VLDL作用于培养的HUVEC ,用细胞酶联免疫吸附试验 (cellELISA)和单核细胞粘附试验检测VCAM 1蛋白在HUVEC的表达 ,用原位分子杂交检测VCAM 1mRNA的表达。结果 正常培养的HUVEC表达VCAM 1,n VLDL和ox VLDL促进HUVEC表达VCAM 1,尤以ox VLDL作用更强。单核细胞粘附试验显示 ,n VLDL和ox VLDL促进单核细胞向HUVEC粘附。结论 天然和氧化极低密度脂蛋白可能通过增强血管内皮细胞表达VCAM 1而促进血液单核细胞粘附于血管壁 ,从而在动脉粥样硬化的早期病变中起重要作用。
Objective To investigate the effect of native and oxidized very low density lipoprotein (n-VLDL, ox-VLDL) on the expression of VCAM-1 in the cultured human umbilical vein endothelial cells (HUVEC).Methods After the HUVEC were stimulated by n-VLDL and ox-VLDL for 24 h, the expression of VCAM-1 protein in HUVEC was detected by cell enzyme linked immunosorbent assay (cell ELISA) and monocytes adhesion test. The expression of VCAM-1 mRNA was detected by in situ hybridization. Results The cultured HUVEC could express VCAM-1. Both n-VLDL and ox-VLDL could enhance the expression of VCAM-1, especially ox-VLDL. The results of monocyte adhesion test revealed that n-VLDL and ox-VLDL could enhance the adhesion of monocytes to HUVECs. Conclusion n-VLDL and ox-VLDL enhanced the recruitment of monocytes to vascular endothelial cells by stimulating the expression of VCAM-1, thus, they may play an important role in early stage of atherosclerosis.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第4期426-428,516,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目 (No 30 30 0 135 )
