医用纳米级Fe_3O_4磁流体的急性毒理学实验研究

Experimental Study on Acute Toxicology of Fe_3O_4 Nano-Magnetic Ferrofluid

目的 检测医用纳米级Fe3 O4磁流体的急性毒性 ,为进一步研究其长期毒性以及载附药物的临床试验打下基础。方法 按不同浓度不同容积分别通过口服法、静脉注射法及腹腔注射法给予小鼠医用纳米级Fe3 O4磁流体 ,观察小鼠的急性毒性反应和主要脏器的病理学改变。结果 小鼠口服半数致死剂量LD50 >2 10 4 8mg/kg ,最大无毒性剂量ED0 为 32 0 10mg/kg ;静脉注射LD50 >4 38 5 0mg/kg ,ED0 为 16 0 0 5mg/kg ;腹腔注射LD50 >15 78 6mg/kg ,ED0 为 32 0 10mg/kg。主要脏器未见明显病理改变。 结论 医用纳米级Fe3 O4磁流体在动物体内的急性毒性很低 ,可以考虑作为药物载体。

Objective To investigate the acute toxicology of Fe 3O 4 nano-magnetic ferrofluid for further use. Methods The acute toxic reaction and the main viscera pathological morphology of mice were evaluated after given Fe 3O 4 nano-magnetic ferrofluid by oral administration, intravenous and intraperitoneal injection with different doses respectively. Results LD 50 (median lethal dose) was >2104.8 mg/kg, ED 0 (maximum non-effect dose) 320.10 mg/kg by oral administration, and degeneration and necrosis of viscera were not found; LD 50 was >438.50 mg/kg, ED 0 160.05 mg/kg by intravenous injection, and degeneration and necrosis were not found either; And LD 50 was >1578.6 mg/kg, ED 0 320.10 mg/kg by intraperitoneal injection. Conclusion Fe 3O 4 nano-magnetic ferrofluid, of which toxicity is very low, may be used as drug carrier.