术前服去氧氟尿苷对胃癌细胞凋亡和增殖影响

The Effects of Preoperative 5'-DFUR Oral Administration on Cell Apoptosis and Proliferation in Gastric Carcinoma

目的 观察术前口服去氧氟尿苷对胃癌细胞凋亡和增殖的影响。方法 采用DNA转移酶dUTP缺口末端标记法 (TUNEL)和增殖细胞核抗原 (PCNA)免疫组织化学染色法 ,观察胃癌患者 (术前化疗实验组和末化疗对照组 ,各n =30 )术后标本的凋亡指数及增殖指数的差异。结果 实验组肿瘤细胞凋亡指数 (3 .68± 1 .2 3) % ,对照组 (1 .1 4± 0 .45) % ,差异具有显著性 (P <0 .0 1 ) ;实验组肿瘤细胞增殖指数 (38.1 3± 8.53) % ,对照组 (47.88± 8.2 8) % ,差异具有显著性 (P <0 .0 1 )。结论 术前口服去氧氟尿苷可以诱导胃癌细胞凋亡 ,有利于胃癌的治疗。

Objective To investigate the changes of apoptotic index(AI) and proliferative index(PI) in gastric cancer following preoperative 5'-DFUR oral administration. Methods 60 surgical specimens of gastric cancer from experimental group (preoperative 5'-DFUR oral administration, n= 30) and control group ( n= 30) were examined for AI and PI respectively by the TUNEL staining method and the Immunohistochemical method of PCNA. Results The AI was significantly higher in pretreated group (3.68±1.23 %) than that in control group (1.14±0.45%), P<0.01. The PI was significantly lower in pretreated group (38.13±8.53%) than that in control group (47.88±8.28%), P<0.01. Conclusions The preoperative chemotherapy with 5'-DFUR can induced effectively cell apoptosis and inhibited cell proliferation in gastric cancer.