增殖细胞核抗原和p27在前列腺增生和前列腺癌组织中的表达及意义

Expression and significance of PCNA and p27 in benign prostate hypertrophy and prostate carcinoma

目的 探讨增殖细胞核抗原 (PCNA)和p2 7蛋白在前列腺增生及前列腺癌组织中的表达 ,及其与肿瘤发生发展的关系。方法 应用免疫组化S P法 ,检测 30例前列腺增生和 37例前列腺癌石蜡包埋存档标本组织中PCNA、p2 7蛋白的表达 ,对其在前列腺增生及前列腺癌不同病理分级、临床分期中的表达进行对比分析和相关性研究。结果 前列腺增生组织的PCNA强阳性表达率(3.3% )与前列腺癌组织 (83.8% )相比 ,差异有显著性 (P <0 .0 1)。前列腺增生组织中p2 7蛋白高表达 (++)率 (70 .0 % )与前列腺癌 (2 7.0 % )相比 ,差异有显著性 (P <0 .0 5 ) ,p2 7蛋白表达与前列腺癌组织的分化程度及临床分期无关 (P >0 .0 5 )。前列腺增生组织中 ,PCNA与p2 7蛋白表达呈负相关 (P <0 .0 1) ;前列腺癌组织中 ,PCNA与p2 7蛋白表达无相关性 (P >0 .0 5 )。结论 p2 7蛋白表达减少或缺失与前列腺肿瘤的发生有关 ,而与恶性肿瘤的进展无关 ,其表达的缺失是前列腺癌重要的阴性预测因子 ;PCNA表达程度不仅与前列腺增生及前列腺癌的发生有关 ,而且与前列腺癌的恶性行为有关 ,PCNA的检测对判断肿瘤的性质。

Objective To investigate the expression of PCNA and p27 in human benign prostate hypertrophy (BPH) and prostate carcinoma (PCa) and their effect on the genesis and progression of the tumor. Methods The paraffin-embedded sections of 30 cases with BPH and 37 cases with PCa were collected. The expression of p27 and PCNA protein were examined by S-P immunohistochemical method. Comparative analysis for BPH and pathological grade and clinical stage of PCa was performed. Results The expression of PCNA in BPH (3.3%) was significantly lower than that in Pca (83.8%, P<0.01). The expression of p27 in BPH (70.0%) was significantly higher than that in Pca (27.0%, P<0.05). The expression of p27 was not correlated with histological grade and clinical stage in Pca (P>0.05). An inverse correlation was found between p27 and PCNA expression in BPH (P<0.01), while no correlation was found in Pca (P>05). ConclusionThe loss or decreased expression of p27 protein may be related to the genesis of benign prostate hypertrophy, but not to the development of prostate carcinoma; the overexpression of PCNA may play an important role in the malignant behavior and progression of prostate carcinoma.