睫状神经营养因子对应激大鼠行为和海马神经元的影响

Effect of Ciliary Neurotrophic Factors on Behavior and Hippocampus Neuron of Stress Rats

本实验采用海马CAl区微量注射法,旷场试验法,Niss1染色法和细胞计数观察100U,500U和1000U的睫状神经营养因子(CNTF)对应激大鼠行为和海马神经元的影响。结果:①急性应激期动物行为活动增加,慢性应激期减少;海马CA1区微量注射CNTF(500U、1000U)可增加慢性应激动物的行为活动。②慢性应激可引起海马神经元退变甚至细胞丢失;CNTF(500U,1000U)可使退变减轻,细胞丢失明显减少。提示CNTF对慢性应激大鼠海马神经元损伤具有保护作用,并可通过海马神经元介导,增加慢性应激动物的行为活动。

The effects of 100U, 500μ and 1 000U ciliary neurotrophic factors (CNTF) on the behavior and hippocampus neuron of stress rats in our experiments were observed and studied by micro-injection of hippocampus CA1, open-field test, Nissl dye and cell count. The results were obtained as follows: (1) animal behavior activity was increased during acute stress and decressed during chronic stress, while injection of CNTF (500U, 1 000U) into hippocampus CA1 region could increase animal behavior activity during chronic stress, and (2) chronic stress could result in degeneration of hippocampus neuron and even in cell loss, while CNTF (500U, 1 000U) could decrease the degeneration and the cell loss obviously. All the results suggest that CNTF can protect the hippocampus neuron from injury and increase animal behavior activity by means of the mediation of hippocampus neuron during chronic stress.