一步自溶法生产ATP的新工艺

A NEW TECHNOLOGY ON THE PRODUCTION OF ATPS BY AUTOLYSIS OF GEOTRICHUM CELLS

新鲜白地霉菌体中核糖核酸(RNA)的含量为5%,白地霉菌体在一定条件下自溶‘菌体中的 RNA 则降解成为5′—核苷酸溶于溶液中,核苷酸收率按菌体中核酸含量计算为80%左右。5′—核苷酸经离交换柱层析法分离得到—磷酸腺苷(AMP)收率为18.5%。由 AMP 制得三磷酸腺苷(AMP)的收率为110%,产品 ATP 含量最高达98.5%,平均在92%左右,经过三年生产实践证实本工艺是有效和切实可行的。

Eresh Geotrichm Candidum Cells contain about 5% of RNA.The cells were autolyzed in borax buffer at 33℃ c for 24 hours.The endogenous RNA was degraded to form 5′—nucleotides.The yield of total 5′—nucleotides is about 80%.The yield of total.ATP making from AMP is about 110%.The highest contents of the ATP' s products are 98.5%.