砷剂对K_(562)细胞凋亡作用的基础研究

Study of the effect As_2O_3 on K_(562 )cells' apoptosis

[目的]研究三氧化二砷对K562细胞凋亡的诱导.[方法]采用人红白血病细胞株K562细胞常规培养,给不同浓度的三氧化二砷,在不同的时间收获细胞,用台盼蓝排染法,DNA荧光染料Hoechst33342荧光染色法,及碘化丙啶(PI)与Hoechst33342共染计数坏死细胞的PI阳性率等方法,检测其对K562细胞的影响.[结果]三氧化二砷能够诱导K562细胞凋亡.并且呈现浓度依赖性和时间依赖性.[结论]三氧化二砷主要以诱导肿瘤细胞凋亡而表现其毒性作用.

[Objective] To observe the effect of As_2O_3 on inducing apoptosis of K_(562)cells. [Methods] K_(562)cells, which is erythroteckemin cell lines, were treated with As_2O_3 of 0,0.2,0.5,1.0,5.0 umol/L in concentration of 24,48,72 hours respectively, then harvested and detected with Hoechst 33342 staining as showing apoptosis cells, PI staining as showing necrosis cells and teypan blue staining as showing living cells.[Results]As_2O_3 inhibited significantly K_(562) cells growth compared with negative control(P<0.01).Its inhibiting ratio was positive relation with the concentration of As_2O_3 and with time. [Conclusion] As_2O_3 is able to induce K_(562) cells into apoptosis.