葡萄籽原花青素的梯度分离及其抗氧活性研究

Studies of Gradient Extraction of Grape Seed Proanthocyanidin and Their Anti-oxidative Effects

目的 :研究葡萄籽原花青素的梯度分离方法及各组分的抗氧化性能。方法 :葡萄籽样品通过乙醇粗提后 ,用不同溶剂梯度分离得到各组分 ,以乙酸乙酯组分为代表确定DPPH·法测定活性的最佳浓度范围 ,再比较各组分自由基淬灭能力。结果 :最佳活性测定样品浓度范围 1.0～ 10 μg/ml,运用此法测得的清除率在 2 5 %～ 80 %之间 ,灵敏度和准确度较高 ;葡萄籽梯度分离各组分清除自由基能力 :乙酸乙酯组分≥丙酮组分 >乙醇组分 >乙醚组分 >乙醇粗提物 >二氯甲烷组分 >石油醚组分 ;其中乙酸乙酯组分和丙酮组分活性尤其突出 ,表明有高活性成分富集其中。结论 :梯度分离葡萄籽原花青素提取物的效果令人满意。

Objective: To explore gradient extraction of grape seed proanthocyanidin and their antioxdative effect. Method: After sample grape seeds were extracted with ethanol, the active components were obtained by application of different organic solvents. Through investigation of the free-radical scavenging effect of ethylacetate components, the optimum concentration range was determined by DPPH method and a comparison was made on the antioxidative effects of the different components from grape seeds. Result: The optimum assay range was 1.0～10 ug/ml with the scavenging rate between 25%～80%. The sensitivity and accuracy of this method were satisfying. The free-radical scavenging effects of different components from grape seed proanthocyanidin came in the following : Ethyl acetate> Acetone> Ethanol> Ether> the primary extracts of ethanol > Dichloromethane> Petroleum ether. Among all components, the activities of Ethyl acetate and Acetone extracts were excellent marked by the active components highly concentrated. Conclusion: Gradient extraction of grape seed proanthocyanidin was very promising.