川芎嗪对P2X_3受体介导的大鼠伤害性兴奋传入的作用

Effect of Tetramethylpyrazine on P2X_3 Receptor Mediated Excitation of Nociceptive Afferents

通过动物痛行为反应(缩足反射)确定局部和鞘内应用川芎嗪(TMP)对ATP等P2X受体激动剂所致大鼠足底急性伤害性行为反应的影响。P2X3受体拮抗剂TNP-ATP(0.3 μ mol／L)明显抑制P2X受体激动剂ATP(1 μmol／L)或α,β-meATP(0.6 μ mol／L)引起的大鼠足底急性伤害性反应。大鼠足底局部应用TMP(0.1-10 mmol／L)剂量依赖性地对ATP(1 μ mol／L)或α,β-meATP(0.6μmol／L)引起的伤害性反应具有抑制作用。鞘内应用TMP(50 mmol／L)对ATP(1μmol／L)或α,β-meATP(0.6 μ mol／L)引起的伤害性反应具有抑制作用。结果表明,TMP可通过阻断P2X3受体介导的伤害性兴奋传入抑制P2X受体激动剂引起的大鼠足底急性伤害性反应。

Using a behavioral study to characterize the effects of TMP administered by intraplantarlly and intrathecally on the acute nociception(flinching) mediated by P2X receptor activation of rat hindpaw. The P2X3 receptor antagonist TNP-ATP(0.3 μ mol/L) significantly depressed the acute nociception mediated by ATP (1 μ mol/L) and α,β -meATP(0.6 μ mol/L) in rat hindpaw. Intraplantar administration of TMP (0.1-10 mmol/L) inhibited the acute nociception in a dose-dependent manner mediated by ATP (1 μ mol/L) or α , [β -meATP (0.6 μ mol/L) in rat hindpaw. Intrathecally applied TMP (50 mmol/L) inhibited the nociception induced by ATP (1 μ mol/L) or α , β -meATP (0.6 μ mol/L) in rat hindpaw. TMP inhibited the nociceptive behaviors induced by P2X3 receptor mediated excitation of nociceptive afferents.