摘要
为研究小鼠胶原性关节炎(CIA)模型的制作并建立评价方法,用皮内注射完全弗氏佐剂的方法建立小鼠CIA模型。容积法检测小鼠足爪炎症的肿胀度并进行评分;分光光度计法检测前列腺素(PGE);3H-TdR掺入法检测脾淋巴细胞增殖反应、白细胞介素-1(IL-1)和IL-2;HE染色法对关节组织作病理检查。结果发现,小鼠足爪的炎症反应强烈,关节肿胀明显;前列腺素产生加剧,T、B淋巴细胞增殖反应、IL-1、IL-2水平显著升高;病理检查关节组织可见其纤维组织增生,水肿,粘液样变,伴有大量炎性细胞浸润,局部血管充血,血管壁增厚。表明皮内注射完全弗氏佐剂建立小鼠CIA模型成功。
To establish the model of collagen-induced arthritis (CIA) and method of evaluation, the mice were injected subcutaneously with CFA (complete Freund's adjuvant) and the change of secondary paw-swelling were observed and scored. The level of PGE was assayed by spectrophotometer; and the 3H-TdR incorporation was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin (IL)-1 and IL-2. Hepatic pathological changes was examined by Hematoxylin-eosin (HE) stain method. The results showed that the inflammation and paw swelling of treated mice increased significantly compared with those of control. Meanwhile, the ConA-induced splenocytes proliferation,PGE, IL-1 and IL-2 production were increased . The pathological examination showed that synovial cell increased ,pannus formed and inflammatory cells infiltrated into synovium. The results indicated that CIA mice model can be established by subcutaneous injection of CFA .
出处
《上海实验动物科学》
2004年第3期149-152,共4页
Shanghai Laboratory Animal Science
