固载表面自组装成环荧光显微成像技术测定阿摩尔量羟甲香豆素(英文)

Fluorescent Microscopic Determination of Hymecromone by a SOR Formed on Glass Slide Support

实验了在辅助成环剂PVA 124存在下,羟甲香豆素的碱性小液滴在经二氯二甲基硅烷处理的疏水性载玻片上,由于接触线的自固定及接触线附近溶剂的挥发,液滴内部产生向外的毛细流将飞摩尔量羟甲香豆素带到接触线上,富集形成环直径小于1 1mm,环带宽小于26 4μm的SOR.环线上溶质的最大荧光强度和羟甲香豆素的浓度成正比关系,当点样体积为0 1μL时,Imax正比于0 1～80 0飞摩尔量的羟甲香豆素(或0 1～40 0×10-8mol/L),检测限达到13 0阿摩尔(或1 3×10-10mol/L)(S/N=3).用本法实测口服药中羟甲香豆素的含量及实际尿液中的回收率在96 0%～104 3%,相对标准偏差为2 7%～3 8%.

A self-ordered ring(SOR) formed by a sessile droplet on glass slide support has developed as a fluorescent microscopic determination of hymecromone of attomole amount. Since the solvent evaporative loss of a sessile droplet on a hydrophobic-treated glass slide, attomole amount of hymecromone materials are carried by the evaporation resulted outward capillary flow to the pinned perimeter of the droplet spot, and deposit there forming a fluorescent self-ordered ring. Depending on the spotted volume of hymecromone solution, different size of SOR with the outer diameter less than 1.1 mm and the ring belt width less than 26.4 μm can be obtained. Data analysis for the imaged SOR by using a digitalized charge-coupled device(CCD) camera showed that the hymecromone molecule across the fluorescent SOR belt section follows a Gaussian distribution, and the maximum fluorescent intensity(I_(max)) was found to be proportional to hymecromone content. When a 0.1 μL droplet was spotted on the solid surface, hymecromone in the range of 0.1-80.0 femtomole(or 0.1-40.0×10^(-8) mol/L) can be detected, and the limit of detection can reach 13.0 attomole(S/N=3, or 1.3×10^(-10) mol/L). With present method, the contents of hymecromone in tablets and urines were satisfactorily detected with the recoveries of 96.0%-104.3% and RSD of 2.7%-3.8%, correspondingly.