固相微萃取-高效液相色谱法测定垃圾渗滤液中的双酚A

Determination of Trace Bisphenol-A in Leachate by Solid Phase Microextraction Coupled with High Performance Liquid Chromatography

本文应用固相微萃取 高效液相色谱法(SPME HPLC)分析了垃圾渗滤液中的痕量双酚A。对SPME的条件如测定模式、pH值、萃取时间、解吸方式、解吸溶剂、解吸时间和HPLC条件进行了优化。建立了SPME HPLC分析垃圾渗滤液中痕量双酚A的方法。方法的线性范围为12.8～192μg/L,相关系数为0.9975,检出限为3.25μg/L(3σ,n=11)。以12.8μg/L的双酚A标准溶液平行测定11次,相对标准偏差(RSD)为4.4%,回收率为94.5%～103.3%。将其用于分析具有垃圾填埋场的渗滤液实际水样,结果十分满意。该方法具有快速、灵敏、简单、无溶剂的特点,适合于环境水样中痕量双酚A的分析。

A rapid determination method for trace bisphenol-A in leachate by solid phase microextraction (SPME) coupled with high performance liquid chromatography (HPLC) was developed. The experimental condition of SPME, such as solid phase microextraction fibers,pH,extraction time, extraction temperature, desorption time, desorption solution,mode and the analytical conditions of HPLC were optimized. The linear range was 12.8～192 μg/L . The correlative coefficient was (0.9975.) The detection limit was 3.25 μg/L(3σ, n=11). The relative standard deviation(RSD)was (4.4%.) The method was used for the determination of trace bisphenol-A in leachate of Qingshan landfill and leachate of Liufang landfill. The recoveries were from 94.5% to 103.3%. This method is fast, convenient, sensitive, solvent free, and suitable for the determination of trace bisphenol-A in leachate.