Eph-B4受体及其配体Ephrin-B2在胃癌组织中的表达及其病理学意义

Expression of Eph-B4 and Ephrin-B2 in Human Gastric Cancer and Meaning of Pathology

目的 :探讨Eph B4受体与其配体Ephrin B2在胃癌组织中的表达及其对血管生成和肿瘤生物学行为的影响。方法 :应用免疫组化SABC法检测 93例胃癌和 30例癌旁组织中Enp B4受体与其配体Ephrin B2的表达及间质微血管密度 (MVD)。结果 :胃癌组织中肿瘤细胞、间质新生血管的Eph B4与Ephrin B2高度表达 ,癌组织中Eph B4与Ephrin B2的阳性表达 (分别为 49.46 %和 50 .54 % )明显高于癌旁组织 (分别为 2 0 .0 %和 2 3 .3 % ,P <0 .0 1 )。Enp B4与Ephrin B2阳性表达组的平均MVD值 (分别为 56 .2 1± 1 5 .3和 62 .41± 1 6 .9)明显高于Enp B4与Ephrin B2阴性表达组 (分别为 32 .2 2± 1 2 .9和 34 .1 2± 1 4 .7,P <0 .0 5和P <0 .0 1 )。此外 ,Enp B4与Ephrin B2表达程度还与胃癌的转移和癌浸润程度密切相关。结论 :Enp B4与Ephrin B2可以促进肿瘤间质的血管生成 。

Objective: The pupose of this study was to investigate the expressions of Eph-B4 and Ephrin-B2 in haman gastic cancer and their effect on tumor angiogenesis and metastasis. Methods:Expression of CD 34,Eph-B4 receptor and Ephrin-B2 ligand were determined by immunohistochemistry in 93 cases of gaseric cancer and 30 cases of adjacent notmal gastric tissue.Results:Measurement of CD 34 expression could be used to describe endothelial cell proliferation and vascularity.Eph-B4 and Ephrin-B2 had higher expression in the gastric cancer specimens than that in adjacent normal mucosa(P< 0.01 ).The MVD value was much higher in Eph-B4 or Ephrin-B2 positive group than that in Eph-B4 or Ephrin-B2 negative group (P<0.05,P<0.01,respectively).In addition,there was a close relatioship between the expression degree of Eph-B4 or Ephrin-B2 and metastasis and the depth of tumor invasion.Conclusion:The results of the current suggest that Eph-B4 and Ephrin-B2 might contribute to the angiogenesis and tumor invasion as well as metastasis in gastric cancer.