内毒素及h-EGF检测与重型病毒性肝炎预后的临床研究

The Clinical Study of Endotoxin and h-EGF Examine in the Severe Hepatitis Prognosis

目的 :为探讨重症肝炎预后的影响因素 ,本文检测了 49例重肝患者血清内毒素和人表皮生长因子 (h EGF)含量变化。方法 :分别采用偶氮显色鲎试验方法及ABC ELISA方法测定血清内毒素和h EGF值。结果 :(1 )死亡组患者血清内毒素水平治疗前高于存活组 ,且与对照组比较P <0 .0 5,而h EGF却相反。 (2 )随着病情进展 ,死亡组内毒素水平升高 ,而h EGF水平有所降低 ,存活组则与之相反。 (3)血清内毒素水平越高 ,病死率越高 ;而h EGF水平越高 ,病死率越低。结论 :内毒素及h EGF分别能反映肝细胞坏死及再生情况 ,其动态检测能较好预测重肝发展趋势 。

Objective: To study the prognosis factor of severe hepatitis, we determined the serum levels of endotoxin and human epidermal growth factor(h-EGF) in 49 patients with severe hepatitis. Methods: Limulus lysate test and ABC-ELISA was used respectively for testing the serum levels of endotoxin, h-EGF. Results: (1) Serum endotoxin levels of death group before therapy are mild higher that of survival group, P<0.05 comparing with control group, but the level of h-EGF is just opposite. (2) As liver disease gradually developed, serum endotoxin levels of death group was increased, h-EGF level was decreased. But those of survival group were opposite. (3) The higher was serum endotoxin level, the higher was mortalities; but the higher was serum h-EGF level, the lower was mortalities. Conclusion: Serum endotoxin and h-EGF can suggest respectively the degree of hepatic necrosis and liver regeneration, the series of determination to them can predict fairly the trend of severe hepatitis, and were used as indices of judging severe hepatitis prognosis.