不同类型早期肠道营养对重症急性胰腺炎大鼠细胞因子的影响

The Effect of Different Types of Early Nutrition Application on Cytokines in Severe Acute Pancreatitis Rats

目的 :评价早期免疫增强型肠内营养对重症急性胰腺炎 (SAP)大鼠血浆细胞因子的影响。方法 :将 1 70只SD大鼠随机分为正常对照组、2 4h组、SAP对照组、传统肠外营养组 (TPN组 )、增强型肠外营养组 (S TPN组 )、传统肠内营养组 (EN组 )和免疫增强型肠内营养组 (S EN组 )。胰胆管逆行注射 2 .5 %牛磺胆酸钠建立SAP模型 ,检测各时点外周血浆TNF α、IL 1 0水平变化。结果 :建模第 4d ,S EN组TNF α、IL 1 0水平较EN组高 ,IL 1 0 /TNF α比值较EN组低 ;第 7d ,S EN组上述指标最低。结论 :早期免疫增强型肠内营养在调节SAP大鼠促抗炎平衡方面优于肠外和传统肠内营养 。

Objective: Compare the effect of early immune-strengthened enteral nutrition application on plasma cytokines in severe acute pancreatitis (SAP) rats. Methods:170 SAP rats were divided randomly into 7 groups:normal group,24 h group,SAP control group,traditional parenteral nutrition (TPN) group,strengthened parenteral nutrition (S-TPN) group,traditional enteral nutrition (EN) group and immune strengthened enteral nutrition (S-EN) group, and SAP was induced in rats by retrograde injection of 2.5% deoxycholate into biliopancreatic.Detecting the levels of TNF-α,IL-10 in plasma at observed times.Results:At 4 days after the operation,TNF-α,IL-10 levels was the lowest,and IL-10/TNF-α was the highest in EN group.In S-EN group, cytokines were higher and the radio was lower than those of EN group;at 7 days, the above items were the lowest.Conclusion:Early immune-strengthened enteral nutrition application is obviously superior to parenteral nutrition and traditional enteral nutrition in modulating the balance of pro-and anti-inflammation,but application prematurely probably go against controlling inflammation.