摘要
对柿基因组DNA的提取方法进行了研究,结果表明,采用提取液中含100mmol/LTris-HCl(pH8 0),20mmol/LEDTA,1 4mol/LNaCl,2%CTAB,1%PVP-40,10mmol/LNa2S2O5及100mmol/Lβ—巯基乙醇(用前加入)的改良CTAB法,可有效去除单宁、酚类、色素等,提取的柿叶片DNA质量和纯度较高,可用于AFLP分析。
The isolation method of persimmon genomic DNA was studied. The results showed that CTAB(cetyltrimethyl ammonium bromide) buffer supplement with 1% PVP-40 (polyvinylpyrrolidone 40), 100 mmol/L β-mercaptoethanal and 10 mmol/L Na_2S_2O_3 was suitable for genomic DNA extraction of persimmon leaves. Assay of the isolated DNA indicated that its purity was very high, with OD_260/OD_280 value between 1.8 and 2.0. The polyhexose, protein and RNA were eliminated completely, thus the extracted DNA can be used for AFLP analysis.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2004年第5期41-45,共5页
Journal of Hebei Agricultural University
关键词
柿树
DNA提取
AFLP分析
persimmon
genomic DNA extraction
AFLP analysis
