绿僵菌高毒力菌株的选育

Screening for High Virulent Mutant Strain of Metarhizium Anisopliae

以金龟子绿僵菌为出发菌株,经物理、化学诱变,确定诱变条件。经诱变初筛时,采用在诱导培养基上测量单菌落大小及透明圈大小的方法,计算其比值,保留比值较大的菌株。再通过复筛测菌株弹性凝乳蛋白酶(Pr1)酶活获得一诱变菌株M6。该突变株产生Pr1活性比出发菌株提高1.10倍,在整个诱变过程中,以出发菌株,代号M原为对照。

The original stains of Metarhizium anisopliae were mutagenized by physical and chemical factors to decide the mutagenesis condition. In the course of screening, the authors measured the dimension of the single colony and transparent zones on the mutagenized media after mutagenizing, calculated it's rate and conserved the bigger stains. A mutant strain M6 was obstained by screening with measuring the strain of Pr1. The Pr1 activity of the mutant was raised by 1.10 times as compared with the original strain .The original strain,code name is M_原 ,as comparision in the mutagenesi.