哈密瓜细菌性果斑病种子带菌的PCR检测

Detection of Acidovorax Avenae Subsp. Citrulli in Melon Seed Using the Polymerase Chain Reaction

利用特异性引物PCR技术,用水或PBST浸提哈密瓜带菌种子中的病原物,浸提液直接作模板就可以扩增出特异性的条带,检测出瓜类果斑病菌(Acidovoraxavenaesubsp.citrulli)的种子带菌情况,种子的浸提时间对检测结果的影响不大,同时查明种皮是种子带菌的主要部位;病瓜种子只有在2粒以上才能检测。采用PCR技术对市售的11个哈密瓜品种的种子带菌情况进行检测,结果检测出8个品种携带瓜类果斑病菌,进一步说明这一方法的可行性和实用性。

Supernatant of melon seeds immersed in water or PBST was directly used as template of DNA amplification of PCR assay. It was shown that the expected DNA brand was amplified and this method could be used to detect Acidovorax avenae subsp. citrulli in seeds. Through PCR assay, it was found that pathogens in seed peel were much higher than those in seed kernel. At least 2 seeds were enough for detection of this pathogen in seeds. With this, 8 of 11 cultivars at market were found having Acidovorax avenae subsp. citrulli in seeds. This demonstrated that PCR assay was feasible and practicable in detection.