不同免疫接种方法评价:多元方差分析方法及其应用

Evaluation of the Effectiveness of Different Vaccination Injecting Methods in Anti-body Production: Introduction and Application of MNOVA (Analysis of Multi-variable Variance)

应用多元方差分析方法评价不同免疫接种方法(交巢与胸肌注射)接种乙肝疫苗与对照的抗-HBsAg的水平,结果表明:(1)在不同时间测量得到的抗-HBsAg水平,在不同处理的鸡只间存在差异,同时在同一处理的不同鸡只间也存在程度不同的差异,对照鸡只抗体含量明显低于其他两种处理,而交巢与胸肌注射之间比较接近。(2)多元方差分析的几种统计量均说明不同免疫接种方法处理的鸡只抗-HbsAg水平存在极显著的差异(F检验,P<0.001);胸肌注射与交巢注射明显优于对照,而这两种方法之间不存在显著差异。(3)不同处理在不同测量时间所获的抗-HBsAg平均水平的最小二乘估计值及其相应的95%置信区间说明:除试验初次测量值外,其它各反应量均存在显著差异,交巢与胸肌注射处理明显优于对照,而这2个处理之间不存在显著差异。(4)应用Bonferonni95%置信区间同时比较不同处理鸡只抗-HBsAg水平测量值之间差异显示:除初次与第2次测量值之外,其余抗-HBsAg水平测量结果之间均存在显著差异,交巢与胸肌注射明显优于对照,而这两种方法之间不存在显著差异。由此可见,不同处理间单一反应量的比较与所有反应量同时进行统计比较,其结果是不一样的。

By one factor factorial experiment with complete randomization design the anti-body concentration in the chick body was repeatedly measured at different time points. With these data the injecting methods were evaluated with MNOVA (analysis of multi-variable variance). The following conclusions were obtained: (1) In the check group the antibody concentration fluctuated around 0.2～0.3 units during the measurement, for the anus and breast injection groups it increased exponentially at the first two weeks after initialization of the experiments then it fluctuated around 2 units. (2) According the outcome of MANOVA there was no statistical significantly difference in antibody concentration between the breast injection group and the anus injection group, but the concentration in both the groups was much higher than that in the check group, But which method is more preferable in practice should be decided by its operability. (3) With the mean estimates the difference test of the antibody concentration among the treatments was done through checking their 95% confidence interval. Except for the first measurement there was significant difference among the treatments. There was higher antibody concentration in the chicks in the anus and breast injection groups than htat in the check, but there was no significant difference between these two treatments. (4) The Bonferonni 95% confidence interval was used to simultaneously test the difference of antibody concentrations among these three groups of chicks. The outcome was a little different from the previous one. There was no statistically significant difference between the first two measurements. And there was significant difference among the other measurements. The anus and breast injection groups had statistically higher antibody concentration than the check, but these two methods produced almost similar results in the measurement of antibody concentrations statistically.