电离辐射致中国仓鼠卵巢(CHO)细胞凋亡的研究

The apoptosis of CHO cells induced by X-rays

为研究X射线致中国仓鼠卵巢(CHO)细胞凋亡,探讨电离辐射的生殖毒性机理,用2—20GyX射线照射CHO细胞株,通过荧光显微镜、AnnexinV/PI双染色法结合流式细胞仪,检测和观察细胞凋亡及其形态学改变。结果表明,X射线照射后24、48、72h均出现细胞凋亡,并随照射剂量增加凋亡细胞数亦增加。但照射后48h和72h细胞凋亡更为明显(p<0.05)。凋亡的CHO细胞在荧光显微镜下,观察到细胞核内染色质的不规则聚集、核固缩和周边化,有的呈月牙形,甚至出现凋亡小体等一系列凋亡细胞形态特征。结果显示电离辐射引起生殖细胞严重的DNA损伤,因无法修复继而导致细胞凋亡。

The work is to study the mechanism of toxic effects on reproductive system and apoptosis of Chinese hamster ovary (CHO) cells induced by X-rays. CHO cell was exposed to X-rays 2 to 20Gy, Apoptosis and morpho- logical changes of the cells were observed by fluorescent microscopy and flow cytometry analyzer with double stain- ing with Annexin V/ PI. The apoptosis could be observed at 24, 48 and 72h after the exposure, but it was more obvi- ous 48 and 72 h after the exposure. Rate of the apoptosis increased along with radiation dose were elevated. Some morphologyical changes, such as irregular agglomerate of chromatins, pycnosis and periphery distribution of nuclei, crescent-moon-like cell, small apoptosis body, were observed. Radiation results DNA damage in the CHO cells, and the demage cannot be repaired, hence the induced cell apoptosis.