摘要
对捕食线虫性真菌———少孢节丛孢菌的DNA提取及18SrDNA基因序列扩增方法进行了研究。结果表明:将少孢节丛孢菌接种于0.4g/L玉米粉液体培养基中,置20℃±1℃温箱中培养2周,其浓缩菌丝经细胞裂解液裂解,可用酚———氯抽提法提取DNA,以该DNA为模板,用真菌特异性PCR引物扩增后,少孢节丛孢菌18SrDNA基因序列的4个PCR扩增片段分别为597bp、555bp、377bp、310bp。
The methods of DNA extraction and 18SrDNA sequence amplification were studied on nematode-trapping fungus——Arthrobotrys oligospora. The results indicated that the concentrated hyphae were lysed by cell lysis solution and their DNA were extracted by phenol-chlorine technique after Arthrobotrys oligospora having been inoculated in 0.4g/L FCM(fluid corn meal) and grown at 20℃±1℃ for 2 weeks. Then, using this DNA template and the specifical PCR primers, the 18S rDNA fragments of Arthrobotrys oligospora were amplified. They were 597bp, 555bp, 377bp,310bp neuleotides respectively.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2004年第3期41-44,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
国家自然科学基金项目(30260081)
