鸡包涵体肝炎中脾脏B淋巴细胞变化规律的研究

STUDY ON REGULATION OF B LYMPHOCYTES OF IBH IN SPLEEN

为了研究鸡包涵体肝炎(InclusionbodyHepatitis,IBH)的发病过程中,机体受IBHV刺激后B淋巴细胞在脾脏中的消长规律。将180只1日龄SPF雏鸡随机分成对照组90只,攻毒组90只,分别在攻毒后1d、3d、5d、7d、9d、12d、15d、20d、25d,心脏采血致死,取其脾脏,分离其淋巴细胞并制成悬浮液,在流式细胞仪上用双染色法检测脾脏中Bu-1+B淋巴细胞。结果表明:脾脏Bu-1+B淋巴细胞1dpi就显著升高(p<0.05)。在病情最重的5dPi、7dPi、12dPi、25dPi(dayspostinfection)均显著高于对照(p<0.05),说明对IBH的免疫起着积极作用。

This research used fowl adenovirus Ⅷ (FAV-8) diluted 1:50 times,and took 0.3ml for a chicken to inoculate 180 SPF chicken of one-day.180 SPF chickens of one-day were randomly divided into control group and infectious group with 90 birds,and killed in 1,3,5,7,9,12,15,20 and 25 days of post infection,spleen were collected and made into the Lymphocyte suspension,and examined the regulation of Bu-1+Lymphocytes by the double staining method with flow cytometer.The result showed that,Bu-1+Lymphocytes of spleen was notably from 1day post infection,and higher (p<0.05) than that of the control at the acutest 5,7,12,25 days post infection,and had a actively action for immune of IBH.