增殖细胞核抗原在急性肝功能衰竭大鼠肝组织中的动态变化

Dynamic changes of proliferating cell nuclear antigen in the livers of rats with acute hepatic failure

目的 :观察增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)在急性肝功能衰竭大鼠肝组织中的动态变化及促肝细胞生长素 (hepatocytegrowth promotingfactors ,HGF)对PCNA表达的影响。方法 :SD大鼠随机分为三组 ,即 :正常对照组、D 氨基半乳糖 (D Galactosamine ,D GalN)组和D GalN +HGF组 ,以D GalN1.2g/kg腹腔注射制备大鼠急性肝功能衰竭模型 ,2h后D -GalN +HGF组大鼠每 2 4h腹腔注射HGF 2mg/kg,分别于第 1天、第 3天、第 5天、第 7天、第 9天、第 11天、第 13天、第 15天用S P免疫组织化学技术检测肝组织中PCNA的表达。结果 :D GalN组大鼠肝组织PCNA标记指数 (PCNAlabelingindex ,PCNA LI)在模型建立成功后第 5天达峰值 ,以后急剧下降 ,在第 15天时达到正常对照组水平 (P >0 .0 5 )。D GalN +HGF组大鼠PC NA LI峰值于第 3天出现 ,比D GalN组早 ,持续时间长 ,且各时间点的PCNA LI均明显高于D GalN组 (P <0 .0 1)。结论 :急性肝功能衰竭时 ,残存肝细胞仍有再生能力 ,但其再生过程受到抑制。HGF可以提高PCNA的表达 ,从而促进肝细胞的再生。

Objective: To observe the dynamic changes of proliferating cell nuclear antigen (PCNA) in t he livers of rats with acute hepatic failure and the influences of hepatocyte gr owth-promoting factors (HGF) on the expression of PCNA. Methods:SD rats were randomly divided into 3 groups: normal g roup,D-GalN group and D-GalN+HGF group. Model for acute hepatic failure was established by injecting D-GalN(1.2 g/kg) into the peritoneal cavity. And all the ra ts in D-GalN+HGF group were injected with HGF(2 mg/kg) per 24 h in the same way afte r 2 hours. The expression of PCNA in liver tissue was detected on the 1st,3rd,5t h,7th,9th,11th,13th and 15th day,respectively by immunohistochemical staining with monoclonal antibody to PCNA. Results:The maximum of PCNA-LI in D-GalN group appeared on th e 5 th day and then sharply declined. On the 15 th day the PCNA-LI reached the level of normal group (P>0.05). But the maximum of PCNA-LI in D-GalN+HGF group appeared on the 3 rd day, and then rapidly declined, too. But the PCNA-LI in the D-GalN+HGF group declined slower and lasted longer than tha t in the D-GalN group. The PCNA-LI at every time point in D-GalN+HGF group was s ignificantly higher than that in the D-GalN group at the same time (P <0.01).Conclusion:The remained hepatocytes of the rats w ith acute hepatic failure can regenerate b ut the regemeration is restrained. HGF can raise the expression of PCNA and so can promote the regeneration of hepatocytes.