海藻酸钙凝胶微球软组织填充材料的制备与体内吸收性的考察

PREPARATION OF THE SOFT TISSUE BULKING AGENT COMPOSED OF CALCIUM ALGINATE MICROSPHERES AND ITS RESORPTION IN VIVO

局部植入组织工程生物填充材料是整形、重建外科治疗相关疾病的有效手段之一。本研究采用静电液滴法制备了不同粒径的海藻酸钙凝胶微球 ,通过一定工艺进一步制备了以海藻酸钙凝胶微球为主体的软组织填充材料 ;通过小鼠背部皮下注射动物模型 ,考察了填充材料在体内的吸收性 ;初步分析了该材料在体内的吸收机理。结果表明 :在阻止或延缓材料在体内吸收方面 ,与凝胶相比 ,微球的作用明显 ;微球的平均粒径与材料在体内的吸收性存在一定的关系 ;海藻酸钠纯化并不显著影响该填充材料在体内的吸收性 ;微球可以完整地从体内回收 ;植入后组织学观察正常。综上所述 。

Soft tissue augmentation with bulking agent is one of the effective methods to treat certain diseases in plastic and reconstructive surgery. A novel soft tissue bulking agent composed of calcium alginate microspheres was prepared by electrostatic droplet method, following the purification of alginate. A 4-week subcutaneous mouse model was used to determine the resorption of the material in vivo and the preliminary mechanism of the resorption was discussed. The experimental results indicated that the calcium alginate microspheres played a key role in the bulking agent to resist the resorption and the degree of the resorption varied with the average diameter of microspheres in bulking agents. In addition, the purification of alginate has no distinctive influence on the resorption. The implanted microspheres were retrieved from the subcutaneous intactly. Histologically, the implants showed biocompatibility in vivo. It is demonstrated that the calcium alginate microspheres have the potential of tissue-engineering bulking agents for soft tissue augmentation.