胡柚皮中黄酮类化合物提取工艺优化研究

Study on Optimum Extracting Technology of Flavonoids in Huyou Peels

目的:常山胡柚(CitrusChangshan-huyouY.B.Chang)为芸香科植物柚〔Citrusgrandis(L.)Os-beck〕与甜橙〔Citrussinensis(L.)Osbeck〕的杂交品种。胡柚皮中含有丰富的具有多种生理活性和较大的药用价值的黄酮类化合物。为进一步将这些黄酮类化合物用于工业化生产并造福人类,对胡柚皮中黄酮类化合物的提取工艺进行优化研究。方法:通过利用二次旋转组合设计的方法,探讨了常山胡柚皮中黄酮类化合物的最佳提取工艺参数。试验结果表明:提取时间、料液比、提取温度和乙醇浓度对提取效率都有显著影响,其中影响最大的因素是提取时间,其次是料液比,提取温度和乙醇浓度对提取效率影响最小。提取效率随温度的升高、乙醇浓度的增加及提取时间的延长而增加,随料液比的增加而降低。结论:最佳提取条件为乙醇浓度90%,料液比1∶5,提取时间115min,提取温度20℃,最大提取量为1.98mg/mL。推荐的工业提取条件以乙醇浓度80%,料液比1∶8,提取温度50℃,提取时间100min为宜。

Objective:Citrus Changshan-huyou Y. B. Chang is a hybrid of Citrus grandisL.Osbeck and Citrus sinensisL.Osbeck. There are abundant flavonoids in Huyou peels. These flavonoids have many biologic activities and pharmacological activities. Extracting technology of flavonoids in Huyou peels was studied in order to improve peoples'lives and use the flavonoids in production. Methods:The optimum extracting technology of flavonoids in Huyou peels was investigated by using quadratic rotational combination designs. Results:The results showed that the extraction was affected by time, ratio of material to solvent, temperature and alcohol contents significantly. Among them extracting time was the primary condition. The ratio of material to solvent was the second important condition. And the effect of extraction was affected less by extracting temperature and alcohol contents than by other conditions. The yield increases as the temperature?the alcohol content and extracting time increased, the yield decreases as the ratio of material to solvent increased. Conclusion:The best extracting conditions are 90% alcohol content, the ratio of material to solvent 1 ∶ 5, extracting time is 115 minutes and at 20℃. The maximal extraction amount of the flavonoids was above 1.98mg/mL. The industrial extracting conditions that we recommended are 80% alcohol content, the ratio of material to solvent 1 ∶ 8, extracting duration 100 minutes and at 50℃.