地面模拟微重力大鼠胃窦部和十二指肠生长抑素免疫反应细胞的变化

A Preliminary Observation on Changes of Somatostatin Immunoreactive Cells in the Gastric Antrum and Duodenum during Simulated Weightlessness in Rats

为了探讨模拟微重力状态下胃肠道生长抑素免疫反应细胞的变化规律,我们用肠卷石蜡切片的免疫组织化学ABC(Avidin Biotin—Peroxidase Complex tech-nique)法,对地面模拟微重力大鼠胃窦部和十二指肠的生长抑素免疫反应细胞进行了初步的观察,并与正常对照动物做了比较。结果表明,微重力动物胃窦部和十二指肠的生长抑素免疫反应细胞较对照动物明显减少。并讨论了这一现象及其与微重力引起的内分泌变化的相互关系。

Weightlessness has been noted to have widespread effects on bodyfunctions.Changes of the cardivascular system,neuro-museular functions,etc.hadbeen documented.Somatostatin(SS)is one of the important bioactive peptides,whichregulates body functions extensively.The majority of SS in the body are produced by SSimmunoreaetive cells in the gastrointestinal tract which are predominantly distributed inthe gastric antrum and duodenum.In order to investigate the effects of weightlessness onthe gastrointestinal SS immunoreactive cells,ABC(Avidin-Biotin- Peroxidase Complex)immumo-cytochemical staining method was applied to the sections of gastric antrum andduodenum of both rats exposed to simulated weightlessness and normal rats to demonstratethe SS cells.Sprgue-Dawely rats were randomly and equally divided into two groups,one for ground-based simulated weightlessness test and the other as control.The rats inground-based weightlessness simulation group were suspended head-down(-30°)by thetail with hind limbs bearing no weight for three months.Food and water were suppliedfreely.The control rats were fed with the same food and water in the same room.Gastricantrum and whole duodenum were dissected out from each rat.Tissues were fixed in 4%paraformaldehyde for 24 h and then embedded in paraffin routinely.Sections of 5 μmwere cut and mounted on gelatine coated slides.Rabbit anti-SS serum(working dilution1:4000)and ABC kit were used in this study.Hsu's standard ABC immumostaining pro-cedure was adopted.The results revealed that the SS immunoreactive cells in rats exposedto simulated weightlessness were significantly reduced.The relationship between this ef-fects and the endocrine alterations caused/induced by weightlessness was discussed.