强肌Ⅰ、Ⅱ号中药改善尾吊大鼠比目鱼肌血流量和肌肉力学特性的作用

Effects of Medical Herbs on Blood Flow and Mechanical Properties of Soleus Muscle in Tail-suspended rats

航天员因失重而产生肌肉萎缩,引起了国内外很多学者的关注,并进行了广泛研究。本文观察尾吊大鼠服用中药后比目鱼肌血流量和肌肉力学特性的变化。实验采用45只SD 品系雄性大鼠,体重240-300g,将鼠按体重分为3组。尾吊大鼠头低位20-25°。三组鼠每天按固定时间分别灌水和强肌Ⅰ、Ⅱ号中药(30g/kg),悬吊15天后,用戊巴比妥钠(4mg/100g)麻醉,用张力、位移传感器测量肌肉力学特性各参数,用标有^(51)Cr 同位素青蛙红血球方法测比目鱼肌的血流量。结果表明:强肌Ⅰ、Ⅱ号中药对尾吊大鼠比目鱼肌血流量、肌肉力学特性有改善作用。其中强肌Ⅱ号效果更明显。比目鱼肌的血流量与等长单收缩、强直收缩张力峰值呈正相关,相关系数分别为0.81、0.84。本结果为防护肌肉萎缩措施提供了实验依据。

45 male Spraque-Dawly rats (240～300g) were randomly as-signed to three groups.The first was control group (fed with water),the second and third groups were fed with medical herbs according to prescriptions No.1,and 2 respec-tively.The rats were tail suspended with heads down 25～30 degree for 15 days.Me-chanical properties of the soleus muscle were determined with tesion and displacementtransducers.Blood flow in soleus muscle was determined with ^(51)Cr labelled frog red cells.The result indicated that blood flow increased in soleus muscle in the second andthird group rats but decresed in the first group rats;peak force of isometric twitch andtetanic contration of soleus muscle increased in the second and third group rats but de-creased in the first group rats.There were corresponding relationships between flood blowand peak force of various kinds of muscle contractions.The correlations were:Y_1=-16.98+705.42x. Y_2=-9.04+1183.76xwhere:x——blood flow in soleus muscle (ml/min.g);Y_1——peak force of isometric twitch (N);Y_2——peak force of isometric tetanic contraction (N).These results provided scientific basis for adopting effective countermeasures againstmuscle atrophy during weightlessness or simulated weightlessness.