人前列腺癌雄激素受体分布特性及血清睾酮水平变化的研究

The study of the androgen receptor profile and changes of level of serum testosterone in human prostatic cancer

采用放射配基结合分析法测定人前列腺癌(PC)穿刺标本的雄激素受体(AR)含量,其胞浆受体(AcR)及胞核受体(AnR)分别为305.70±461.68,363.04±391.44pmol/g蛋白,与36例前列腺增生症(BPH)及9例正常前列腺组织含量相比均有显著性差异,显示前列腺癌的雄激素依赖性。22例前列腺癌患者中未转移组的AnR/AcR比值明显大于转移组,提示前列腺癌转移过程中AnR向胞浆转移的可能性。前列腺癌患者的血清睾酮水平明显低于前列腺增生症患者及正常人。放射自显影术显示雄激素受体定位以前列腺上皮细胞核为主,表明雄激素通过与核内受体结合调控肿瘤发生发展的可能性。

The androgen receptors in biopsy specimens of 22 cases of human prostatic cancer(PC) were studied by radioligand binding assay. The cytoplasmic androgen receptor (AcR) and nuclear androgen receptor(AnR) densities wewe 305.70±461.68 and 363.04±391.44 pmol/g protein respectively, both were significantly higher than those of 36 benigh prostatic hypertrophy (BPH) and 9 normal prostate(NP). Among the prostatic cancers, the AnR/AcR ratios were significantly different between metastatic and primary cancers. This result suggested that there might be migration of AR from nucleus to cytosoi in the process of metastasis. The serum testosterone studied by RIA method are significantly lower than that of BPH and NP. Thawmounted autoradiography demonstrated that AR were mainly located in epithelial cells of the glandular tissue of prostate.