酸樱桃组织培养及快速繁殖技术研究

On the Technology of Tissue Culture and Rapid Propagation of Prunus cerasus

以芽苞、茎尖和茎段为外植体,在MS培养基中加入不同浓度的6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)、吲哚丁酸(IBA),对酸樱桃(Prunus Cerasus)进行了组织培养快速繁殖技术的研究.结果表明:以MS+6-BA2.0mg/L+IBA0.1mg/L为酸樱桃最适宜增殖培养基,增殖率为4～5倍.壮苗培养基为不加激素MS培养基.适合诱导生根的培养基为1/2MS+IBA1.0mg/L,生根率为93%.以蛭石+珍珠岩体积比1∶2为培养基的组培苗移栽成活率最高,达到86%以上.

The technology of rapid propagation of Prunus cerasus was studied by tissue culture. The culture medium is MS supplemented with different concentrations of 6-BA, NAA, IBA respectively or commixture. Explants are new buds, tips of stem and sections of stem respectively. MS+6-BA?(2.0?mg/L)+IBA?(0.1?mg/L) is good medium for Prunus cerasus propagation and propagation rate is 4～5?times. Arbustive buds are cut as individual plant and planted in MS medium without hormone. The medium producing adventitious root is (1/2?MS)+IBA?(1.0?mg/L). Inducing root rate is 93%. Survival rate of tube-seeding is the highest transplanting in substrate which is vermiculite and pearlite(1∶2). Survival rate of transplanted tube-seeding is 86%.