摘要
目的 :建立一种简便、易行和高效的胰岛分离纯化方法 ,以减少对胰岛细胞的损伤 ,获得高质量的胰岛组织。方法 :选用SD大鼠经胆总管原位灌注胶原酶液 ,分离消化胰腺组织 ,用淋巴细胞分离液 (histopaque 10 77)纯化胰岛细胞。结果 :可收获大量的高纯度胰岛 ,纯度达 95 %以上 ,体外葡萄糖刺激实验表明胰岛对刺激反应良好 ,将胰岛移植于糖尿病小鼠体内可短期纠正其高血糖状态。结论 :应用单一密度淋巴细胞分离液纯化大鼠胰岛的方法是一种操作简单、价格低廉、重复性好的大鼠胰岛纯化方法。
Objective:Establishing a kind of convenient and effective method to isolate and purify islets in order to decrease injury and obtain islets with high quality.Methods:Pancreas of SD rats were perfused with collagenase through choledochus, then the pancreas were isolated and digested. Lymphocyte (Histopaque-1077) was used to purify the digested pancreas.Results:This method could obtain a large number of rat iselets with purity >95%. The isolated islets had a correct response upon glucose stimulation in vitro. When the iselets were transplanted into diabetic mice the high blood glucose level could be decreased. Conclusion:Using lymphocyte to purify islets of rat is a kind of convenient, effective, cheap, and repeatable method.
出处
《军医进修学院学报》
CAS
2004年第4期299-300,共2页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金资助项目 ( 3 95 70 72 5 )
