凋亡调节蛋白在手术切除的原发性非小细胞肺癌中的表达

Expression of apoptopsis regulatory proteins in primary resected non-small-cell lung cancer

目的检测凋亡调节蛋白抑癌基因p53和Bcl-2家族在非小细胞肺癌(NSCLC)标本中的相关性和预后意义。方法采用免疫组化法用特异性针对人体的p53和Bcl-2家族的抗体检测这些凋亡调节基因在50例存档的、手术彻底切除的NSCLC患者标本中的表达情况,分析它们之间的相互关系,并追踪患者的生存情况,分析其表达的预后意义。结果(1)31例(62%)的NSCLC表达抑癌基因p53,典型的p53染色免疫阳性是细胞核染色。典型的Bcl-2家族蛋白呈胞浆染色,少数也可以呈胞核染色阳性。(2)p53的表达与患者的吸烟史呈正相关(P<0.05),p53与Bcl-2的表达呈显著的正相关(P<0.05),p53与Bax的表达之间呈显著的负相关(P<0.05)。(3)p53的表达阴性的肿瘤患者没有发生远处转移,p53的表达与远处转移的发生呈正相关(P<0.05)。Bcl-2的表达与原发性NSCLC手术切除患者的生存时间(P<0.05)呈负相关。结论抑癌基因p53和Bcl-2家族蛋白在NSCLC中的表达等都很常见。p53突变可能使Bcl-2表达增加,Bax表达减少而增加远处转移的风险。

Objective We performed immunohistochemistry to evaluate the apoptopsis regulatory proteins of association and prognostic significance of the tumour suppressor p53 and the Bcl-2 family Bcl-2 ,Bax and Mcl-1 in the samples of human primary non-small-cell lung cancer. Methods We used antibodies specific for the human p53, Bcl-2 ,Mcl-1and Bax proteins to examine the expression of these apoptosis-regulating genes in 50 archival specimens of patients with radically resected non-small-cell lung cancer (NSCLC). Their relations with eath other were comparatively analyzed. We also followed up the outcome of the patients and comparatively analyzed prognostic significance of these proteins. Results(1)The tumour suppressor p53 was present in 31(62%) of the cases evaluated, it typically expressed in the cytoplasm. The expression of Bcl-2 family were not only present in the cytoplasm, they also present in the nucleus. (2) The expression of p53 associated with a history of smoking (P0.05) , there was significant positive association of p53 and Bcl-2 expression (P<0.05), while the expression of p53 was inversely related to Bax(P<0.05). (3)No metastase was observed in patients with p53-negative tumours, p53 overexpression and metastase was positively related (P<0.05).The expression of Bcl-2 had a negative influence on survival in this population of primary resected NSCLC patients (P<0.05). Conclusion The expression of the tumour suppressor p53 and the Bcl-2 family proteins in NSCLC are frequent. p53 mutation may increase the expression of Bcl-2, while decrease the expression of Bax, through which increase the risk of metastatic recurrence in NSCLC.