老年冠心病患者血小板蛋白激酶C、蛋白激酶C抑制剂及红细胞蛋白激酶C活性变化的研究

The inhibitor activity on platelets and activity on erythrocytes of protein kinase C from elder patients with coronary heart disease

目的 研究蛋白激酶C(PKC)在冠心病的发生、发展中的作用。方法 测定 87例心绞痛 (AP)患者、91例急性心肌梗死 (AMI)患者、90名健康对照 (HC)者的血小板胞膜、胞浆PKC、胞浆PKC抑制剂 (PKCI)活性和红细胞胞膜、胞浆PKC活性。结果 AP组和AMI组血小板胞膜中PKC活性明显高于HC组 ,其中AP组与HC组比较 ,增高 6 2 .6 % (P <0 .0 1) ;AMI组与HC组比较 ,增高 4 1.2 % (P <0 .0 1) ;AP组与AMI组比较 ,增高 14 .2 % (P <0 .0 5 )。AP组和AMI组血小板胞浆中PKC活性明显低于HC组。其中AP组与HC组比较 ,下降 36 .6 % (P <0 .0 1) ;AMI组与HC组比较 ,下降 2 3.9% (P <0 .0 1) ;AP组与AMI组比较 ,下降 16 .7% (P <0 .0 5 )。AP组和AMI组血小板胞浆中PKCI活性明显低于HC组。其中AP组较HC组下降 5 2 .9% (P <0 .0 1) ,AMI组较HC组下降 2 6 .0 % (P <0 .0 1)。AP组及AMI组红细胞胞膜中PKC活性明显高于HC组。其中AP组与HC组比较 ,增高 33.6 % (P <0 .0 1) ;AMI组与HC组比较 ,增高 31.8% (P <0 .0 1)。AP组及AMI组红细胞胞浆中PKC活性明显低于HC组 ,AP组较HC组降低 2 7.2 % (P <0 .0 1) ,AMI组与HC组比较 ,降低 2 1.9% (P<0 .0 1)。

Objective To study the relationship between protein kinase C(PKC) and coromary heart disease. Method To measure the activity of PKC with its inhibitor (PKCI) on Platelets and activity on erythrocgtes from 87 patients with angina pectoris (AP), 91 patients with acute myocardial infarction (AMI), and 90 healthy control patients (HC), respectively.Results The activities of PKC in membrance of platelet from AP and AMI were higher than those of HC. Those of AP rise by 62.6%(P<0.01) than those of HC. Those of AMI rose by 41.2%(P< 0.01) than those of HC. While those of AP rose by 14.2% (P<0.01) than those of AMI. The activities of PKC in Cytosol of platelet from AP and AMI were lower than those of HC. Those of AP decreased by 36.6% (P<0.01) than those of HC. Those of AMI decreased by 23.9%(P<0.01) than those of HC. While those of AP decreased by 16.7% (P<0.05) than those of AMI. The activities of PKCI in Cytosol of platelet from AP and AMI were lower than those of HC. Those of AP decreased by 52.9%(P<0.01)than those of HC. Those of AMI decreased by 26.0%(P<0.01)than those of HC.The activities of PKC in membrane of erythrocytes from AP and AMI were higher than those of HC.Those of AP rose by 33.6%(P<0.01)than those of HC. Those of AMI rose by 31.8%(P<0.01) than those of HC. The activities of PKC in cytosol of erythrocytes from AP and AMI were lower than those of HC.Those of AP decreased by 27.2%(P<0.01) than those of HC. Those of AMI decreased by 21.9%(P<0.01) than those of HC.Conclusion PKC is likely to be associated with the pathogenesis of Coronary heart disease.