液氮保存的同种主动脉瓣叶内皮细胞活性的变化

Changes of Cryopreserved Endothelial Viability of Homograft Aortic Valve Leaflets

目的 :研究液氮不同保存时间对人类同种主动脉瓣叶内皮细胞活性的影响 ,探讨同种瓣的有效使用期限。方法 :取健康成年男性、脑死亡6h以内同种带瓣主动脉管道 ,灭菌、程控降温至 -60℃后液氮保存。将主动脉瓣叶随机分为10组存储不同时间。应用流式细胞仪检测上述标本的内皮细胞活性 ,比较各组之间的差异。结果 :新鲜冷冻组和液氮保存2年以内的瓣叶内皮细胞存活率与新鲜组无明显差异 ,而液氮保存3～8年的同种主动脉瓣叶内皮细胞死亡率明显增加 ,差别有统计学意义 (P<0.05)。结论 :液氮保存3年以上的同种带瓣主动脉内皮细胞能否应用于临床值得商榷。

Objective:To study the influence of cryopreserved time on endothelial viability of human aortic valve homograft(AVH)and the useful-life of human homograft aortic valves.Methods:The hearts were acˉquired from healthy adults who died within6hours.The AVH were cryopreserved after controlled rate refrigˉeration.All AVH were randomly divided into10groups.The endothelial viability of AVH was detected by Flow Cytometry(FCM).Results:There was no significant differences in endothelial viability between fresh AVH and those cryopreserved less than2years.The endothelial mortality rates of AVH cryopreserved between3to8years markedly increased than that in fresh group(P<0.05).Conclusion:It is deliberated for AVH cryopreˉserved more than3years used in clinic.