婴儿双歧杆菌发酵培养基组成的优化

Studies on Fermentative Medium Optimum Formulation of Bifidobacterium Infantis

针对婴儿双歧杆菌的营养需要,分别采用单因素试验和正交试验,优化培养基成分及用量。得出最佳发酵培养基(CPT)配比:大豆蛋白胨1.67%,酪蛋白胨0.83%,乳糖0.5%,酵母浸出粉0.5%,低聚糖0.7%,胡萝卜汁15%。最后测定婴儿双歧杆菌在发酵培养基中的生长曲线,并同时测定pH和吸光值的变化,确定发酵终止时间为12h,此时菌数可达2.18×109cfu/ml。

According to the nutritional needs of B.infantis, the ingredient and dosage of medium were optimized by single factorexperimentation and quadratic design respectively. The optimum conditions of fermentative medium were as follows: 1.67%soya peptone, 0.83% casein peptone, 0.5% lactose, 0.5% yeast extract, 0.7% oligofructose and 15% carrot juice. Growth curveswith optimized fermentative medium were determined, with the change of pH and optics density. According to the growthcurve, the terminal time was 12h with B.infantis reeching 2.18×109cfu/ml.