摘要
在传统制备超氧化物歧化酶的基础上,进行工艺改进。直接用血块,采用机械破碎法破膜,热变性及两次乙醇-氯仿沉淀除杂蛋白,然后丙酮沉淀,按照此工艺分离提取获得高纯度的Cu,Zn-SOD,比活达到6988U/mg·pro。
A new procedure is designed based on the conventional method. Higher purity Cu,Zn-SOD is obtained by thermaldenaturalization, twice ethanol-chloroform to remove other protein, acetone precipitation. Its specific activity is 6988U/mg·pro.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第8期121-124,共4页
Food Science