葛根中异黄酮的提取与纯化工艺研究

Study on the Optimum Extraction and Purification Technology of Isoflavonoid of Pueraria Lobata

本文研究了葛根中异黄酮的最优浸提条件和最佳工艺参数,并对提取液的纯化条件进行了研究,最后对纯化产物进行了定性鉴定。结果表明:乙醇浸提法的最佳提取工艺参数为:料液比1:8、75%乙醇、温度70℃、提取时间2.0h;水提取法的最佳浸提工艺参数为:料液比1:10,浸提时间3h,浸提温度100℃,提取次数3次。葛根中异黄酮的纯化采用氯化钠盐析法,醇提液的纯化产率可达4.96%,产品含量为33.84%;水提液的异黄酮产率为6.45%,含量为10.50%。

The optimal extraction and purified technology of pueria isoflavonid were studied in this experiment. The resultsshowed that the optimum conditions of the isoflavone extraction rate of material to using alcohol as 1:8, 75%alcohol for 2h andat the temperature of 70℃. The optimum conditions of the isoflavone extraction by using water: rate of material to water as1:10 for 3h and at the temperature of 100℃ with 3 times. The results of purifying with NaCl showed that the yield of alcoholextracted isoflavonoid was over 4.96%,and the content of isoflavonoid was isoflavonold 33.84%, the yield of water extractedisoflavonoid was over 6.45%,and the content of isoflavonoid was 10.50%.