骨关节结核病灶结核菌L型状况的观察

Study on L-form Mycobacterium bacilli in the lesions of osteoarticular tuberculosis cases

目的 探讨骨关节结核病灶中 ,结核菌L型的存在状况。方法 样本均采自骨科住院病人的脓液、肉芽、干酪物等 ,经碱液处理后 ,分别进行直接涂片 ,并接种在罗氏培养基上、Bactec培养液及结核菌L型培养液中 ,以查找结核杆菌及结核菌L型。结果 在 6 0例中 ,查到结核菌L型 11例(18.3% ) ,涂片阳性 9例 (15 % ) ,罗氏培养阳性 3例 (5 % ) ,快速培养阳性 12例 (2 0 % )。结论 对骨关节结核病灶进行结核菌培养的同时 ,加做结核菌L型检查是十分必要的。

Objective To explore the exist of L-form Mycobacterium bacilli in the lesions of bone and joint tuberculosis.Methods After N-Acetyl-L-Cystein-Sodium Hydroxide (NALC-NaOH) decontamination and Z-N stains,the specimens collected from the lesions were inoculated in L-J culture media,Bactec 460 and L-form broth media for detecting the Mycobacterium bacilli and L-form of Mycobacterium bacilli.Results The L-form of Mycobacterium bacilli were found in the lesions of 11(18.3%) osteoarticular tuberculosis patients.The smear positive rate of smear and L-J culture positive were 9 cases (15%) and 3 cases (5%) respectively.The positive rate of Bactec 460 was 12 cases (20%).Conclusion It is necessary to make L-form Mycobacterium bacilli examination for retreatment tuberculosis patients.