幼犬深低温低流量体外循环手术脑保护方法实验

Brain protective methods for young dog during deep hypothermic low-flow cardiopulmonary bypass

目的 探讨尼莫通在长时间深低温低流量体外循环手术中对幼犬中枢神经系统的保护作用。方法 12只 6个月健康杂种犬随机分为两组。对照组 ,进行 2h深低温低流量体外循环心脏手术 ;研究组 ,在体外循环同时颈动脉持续以 15 μg/ (kg·h) 的速度给予尼莫通。两组动物分别检测不同时点颈内静脉血的丙二醛 (MDA)、总抗氧化能力 (TAOC) ;每组动物术后检测脑组织MDA和TAOC。结果 每只动物不同时点颈内静脉血MDA含量及TAOC活性有差别 ;研究组动物不同时点颈内静脉血MDA含量和TAOC活性与对照组差异有显著性 (P <0 0 5 )。研究组动物脑组织MDA含量和TAOC活性与对照组比较差异有显著性 (P <0 0 5 )。结论 钙拮抗剂尼莫通是一种有效的脑保护药物 ,在幼犬深低温低流量体外循环中综合运用尼莫通可起到较好的脑保护作用。

Objective To investigate the brain protective effects of nimotop on the brain of young dogs during the course of deep hypothermic low-flow cardiopulmonary bypass. Methods Twelve healthy six-month-old mongrel dogs were randomly assigned into two groups, with six dogs in each group. In control group, cardiopulmonary bypass with deep hypothermic low-flow was used for 2 hours. In experimental group, the basic steps were as same as above, but before cardiopulmonary bypass, the intrajugular artery was punctured and nimotop was injected at 15 μg/(kg·h) through the whole experiment. The concentration of MDA and TAOC in blood sample from jugular vein at different time was examined after the experiment. The contents of MDA, TAOC of brain tissue were measured after operation. Results The concentration of MDA and TAOC were significantly different among different time points in the same dog. The concentration of MDA was different between the control group and experimental group, and the concentration of TAOC was also between the control group and experimental group. The content of MDA of the experimental group was(5.83±1.99)nmol/(mg·prot), significantly lower than the that of control group(11.27±4.51)nmol/(mg·prot)(P<0.05);the content of TAOC of experimental group was(1.762±0.717)U/(mg·prot), significantly higher than that of control group(0.983±0.026)U/(mg·prot)(P<0.05).Conclusion Nimotop has brain protective effects. It is an effective brain protective method to use nimotop during the course of deep hypothermic low-flow cardiopulmonary bypass.