摘要
目的 探讨糖尿病胃肠功能紊乱大鼠胃肠道自主神经病变的靶位。 方法 应用四氧嘧啶 糖尿病大鼠模型 ,对其胃肠道多个部位的 β2 肾上腺素能受体 (β2 AR)与 5 羟色胺受体 (5 HTR)的数量进行免疫组化检测。 结果 糖尿病大鼠胃窦与结肠的 5 HTR显著增多 ;糖尿病大鼠黏膜内分泌细胞的 β2 AR在肠道均有减少。 结论 胃肠道的 β2 AR与 5 HTR的数量在糖尿病胃肠功能紊乱时发生了改变 ,这种改变很可能是糖尿病胃肠功能紊乱的发病基础。
Objective To clarify the autonomic nerve d isorder of gastrointestinal tract in diabetic rat with gastrointestinal dysf unction. Methods Diabetic SD rats aged at 12 weeks were rand omly divided into two groups: group A(diabetic), group B(control). Using allox an-prepared diabetic rat model with gastrointestinal dysfunction, the quantity of β 2-adrenergic receptor(β 2-AR) and serotonin receptor(5-HTR) of the g astrointestinal tract were detected by immunohistochemical stain. Resul ts The quantity of 5-HTR in myenteric nerve plexus at gastric antr um and colon were increased (χ2=26.40, P<0.01; χ2=11.01, P<0.01, sepa rately). The quantity of β 2-AR of mucous membrane and myenteron at small int estine and bowel in diabetic rats were all decreased (χ2=11.40, P<0.01; χ 2=7.71, P<0.01; χ2=27.81, P<0.01; χ2=18.71, P<0.05, separately). Conclusions The quantity of β 2-AR and 5-HTR in gastrointest inal tract are changed in rat with diabetic gastrointestinal dysfunction. these changes may be the pathogenesis foundations.
