咬合创伤大鼠三叉神经节PN_3及NaN mRNA的表达

Expression of PN3 and NaN in trigeminal ganglion during occlusal trauma in rat

目的 观察PN3 和NaN 2种钠通道在咬合创伤致口面部慢性疼痛中的变化和作用。方法 应用RT PCR方法 ,观察咬合创伤大鼠及空白对照大鼠双侧三叉神经节中PN3 和NaNmRNA的表达。结果 空白对照组及实验组对照侧均有PN3 、NaNmRNAs表达。空白对照组两侧亮度值水平接近。咬合创伤实验动物中 :咬合创伤侧PN3 亮度均数值为 94 ,NaN亮度均数值为 99;非咬合创伤侧PN3 亮度均数值为 12 0 ,NaN亮度均数值为 114。咬合创伤侧三叉神经节中PN3 、NaNmRNAs的凝胶电泳图像亮度值高于非咬合创伤侧。结论 咬合创伤刺激上调PN3 、NaNmRNAs表达 。

Objective To investigate changes of two sodium channels, PN 3 and NaN, during oroficial pain by occlusal trauma in rat Methods Expressions of PN 3 mRNA and NaN mRNA in trigeminal ganglion were tested during various periods of persistent occlusal trauma with reverse transcription polymerase chain reaction (RT PCR) Results In groups, including control,occlusal trauma groups, PN 3 mRNA and NaN mRNA were all expressed in trigeminal ganglion neurons In the control group, there were similar density values bilaterally In the occlusal trauma group, the density values in gel electrophoresis of PN 3 mRNA and NaN mRNA on the intervention side were slightly greater than those on the control side Conclusions The stimulation of occlusal trauma upregulates expressions of PN 3 mRNA and NaN mRNA, which suggests the signal occurring and conduction of chronic pain by occlusal trauma have the same molecular mechanism of sodium channel as inflammatory pain