自体成骨细胞的诱导培养及其生物学特性

Osteoblast induction and cultivation from bone marrow and its biological characteristics

目的 诱导培养来自自体骨髓间充质干细胞的成骨细胞或成骨细胞的前体细胞 ,进行自体成骨细胞异体骨复合移植实验研究。方法 抽取动物骨髓、抗凝稀释 ,以适当条件培养诱导 ,分化为成骨细胞并分析细胞生物学特性。成骨细胞生长在同种异体骨制成的载体上 ,形成复合物 ,植入人工缺损处 ,观察骨修复情况。结果 可以诱导培养出大量的成骨细胞或成骨细胞的前体细胞。自体成骨细胞异体松质骨复合物修复骨缺损的效果明显优于对照组。结论 本实验为自体成骨细胞异体骨复合移植的临床应用提供一个简单有效的方法。

Objective To induce and cultivate osteoblast (OB) from bone marrow mesenchymal stem cells (MSC) for transplatation of an autoosteoblast and allogeneic bone complex. Methods Bone marrow was collected and diluted with anticoagulant. The cells were cultured and induced into OB in conditional media. OB differentiation, growth and biological characteristics were observed, OBs were plated and grew on allogeneic bone slices, which were transplanted to bone defects. Results OB were induced from MSC and grew abundantly. The transplantaion of auto-OB and allogenic bone complex to repair bone defects had a maruedly better result than that in control group. Conclusion This experiment provides a simple and effective method for clinical use of allogeneic bone complex transplantation.