淫羊藿抑制颌骨骨吸收的体外实验研究

Effect of Epimedium grandiflorum Morr on osteocalstic bone resorption

从乳兔长骨分离出培养破骨细胞 ,并与人下颌骨骨磨片体外共同培养 ,分别加入不同浓度 (0 ,0 15 ,1 5和 15mg/ml)的淫羊藿注射液 ,倒置相差显微镜观察破骨细胞形成的骨吸收陷窝 ,并对陷窝数目和面积进行图像分析 ,探讨淫羊藿对破骨细胞性颌骨骨吸收的影响。结果显示 ,与对照组相比 ,淫羊藿 3个实验组 (0 15 ,1 5和 15mg/ml)均能有效抑制破骨细胞在下颌骨片上形成的吸收陷窝的数量和面积 ,并且抑制作用呈剂量依赖性递增。

Osteoclasts were isolated from long bones of newborn rabbits and cultured on human mandible slices in vitro. Each bone slice was placed in a 24-well culture plate containing various concentrations of Chinese herb Epimedium grandiflorum Morr in α-MEM.The effect of Epimedium grandiflorum Morr on bone resorption lacunae generated by osteoclasts was observed under phase-contrast microscope. The results showed that Epimedium grandiflorum Morr various concentrations(0.15 mg/ml,1.5 mg/ml,15 mg/ml)have significant inhibitory effect, which is increased with concentration of the drug.