摘要
目的 通过对小鼠骨髓干细胞体外培养的观察 ,研究辛伐他汀在骨髓基质干细胞向成骨细胞定向分化过程中的作用。方法 取雄性 6周ICR小鼠股骨骨髓基质细胞进行原代和传代培养 ,应用组织化学及vonKossa方法检测细胞碱性磷酸酶染色和细胞外基质矿化 ;在细胞培养早期加入辛伐他汀 (实验组 )或保持基础培养条件 (对照组 ) ,应用半定量RT PCR方法分别检测两组Ⅰ型胶原蛋白 (COL1)、碱性磷酸酶 (ALP)、转录因子CBFA1和Osterix(OSX)在成骨细胞分化过程中的表达。结果 小鼠骨髓基质细胞经体外诱导后分化为具备碱性磷酸酶活性和矿化细胞外基质的成熟成骨细胞。实验组COL1、ALP和CBFA1表达在细胞培养第 3,5天均高于对照组 ,OSX表达差异不明显。结论 辛伐他汀在成骨细胞分化过程中促进其相关基因的表达。
Objective To study the effect of simvastatin on osteoblast differentiation by using a mouse bone marrow culture system.Methods Bone marrow cells were derived from 6-week-old ICR mice. Dexamethasone was initiated in the cultures. Alkaline phosphatase staining and von Kossa staining were used to assess osteoblast differentiation. Semi-quantitative RT-PCR was perfermod for evaluating the expressions of collagen type I (COL1), akaline phosphatase (ALP), CBFA1, and Osterix on culture days 3 and 5, in the simvastatin-(treated) gron and blank control group.Results Cells in each group were differentiated into osteoblast lineage, as shown by alkaline phosphatase and von Kossa stainings. The expressions of COL1, ALP and CBFA1 in the treatment group was higher than those in the control group.Conclusion Simvastatin enhances the expression of osteoblast-differentiation-related genes.
出处
《中国骨质疏松杂志》
CAS
CSCD
2004年第3期319-321,329,共4页
Chinese Journal of Osteoporosis
基金
国家自然科学基金资助项目 ( 3 0 0 70 3 67)
