荣骨颗粒对卵巢切除后骨质疏松大鼠骨形态计量的影响

Rong Gu Granules offection to the histomorphometric analysis of ovariectomy osteoporotic rats

目的 探索补肾中药荣骨颗粒对去势大鼠骨质疏松症的防治作用。方法 摘除大鼠双侧卵巢造成骨质疏松症模型 ,分别灌服大、中、小剂量的荣骨颗粒 ,并与己烯雌酚和骨疏康组作对照 ,3个月后 ,进行骨形态计量测定和分析。结果 大中剂量的荣骨颗粒能使切除卵巢大鼠骨小梁体积百分比显著增高 ,骨小梁吸收表面百分比、骨小梁形成表面百分比、活性生成表面百分比、骨小梁矿化率、类骨质平均宽度及骨皮质矿化率均明显降低。结论 大、中剂量的荣骨颗粒对卵巢切除后的大鼠骨质疏松症具有一定的治疗作用 ;小剂量荣骨颗粒无明显作用 ,而大剂量则优于中剂量组的治疗作用。

Objective To study the effection of Rong Gu Granules in the treatment of Osteoporosis. Methods Seventy-nine Sprague-Dawley rats were randomly divided into seven group: one normal control group, one model group, three Rong Gu Granules treated groups and two positive groups. osteoporotic rat models were established by ovariectomy. After 3 months treatment, all animals were killed. The proximal tibia were processed to undercalcify sections for histomorphometric analysis. Results The ovariectomy rat osteoporosis model is the one that rate of bone resorption is larger than that of bone formation, and bone transformation become faster than before. Rone Gu Granules can make this situation reverse: bone mass indexes of Rong Gu Granules treated groups are larger than that of model group, meanwhile, the bone resorption indexes decrease and the bone formation indexes increase. Conclusions Rong Gu Granules can inhibit the bone loss and protect the bone. It exerts dual effection in restraining bone absorption and bone formation, It can also reduce bone transformation and increase bone mass.