半导体激光诱导的鼠脉络膜新生血管模型研究

Study on the Experimental Model of Diode Laser-Induced Choroidal Neovascularization in Rat

目的评价半导激光诱导BrownNorway (BN)大鼠的脉胳膜新生血管 (choroidalneovascularization ,CNV)模型的可行性。方法用半导体激光 (波长 810nm ,曝光时间 0 .1s,光斑 10 0 μm ,能量 10 0～ 14 0mV)对 10只BN大鼠视乳头附近的视网膜进行光凝 ,每眼 10个激光点 ,光凝后 2小时 ,3天 ,1、2、4周行病理组织学检查 ,观察光斑区CNV的产生及变化过程。结果激光照射后 2小时及 3天无DNV形成 ,激光光凝后 1周出现CNV。且病理切片证实激光光凝后 1、2、4周CNV发生率分别为 35 7%、72 2 %、70 6 %。结论通过半导体激光诱导BN大鼠CNV模型是可行的有效的 ,特别是针对CNV的药物干预及相关基因水平研究。

Objective To evalute the feasitility of inducing choroidal neovascularization in Brown Norway(BN)rat by diode laser. Methods Ten anesthetized BN rats recieved a series of 10 diode laser lessions per eye.Laser photocoagulations were applied to each eye between the major retinel vessels around the optic disc with a diode laser photocoagulator and slit lamp delivery system at a spot dize of 100μm with duration of 0.1 second,power of 100～140mV and wavelength 810nm 2 hours,3 days,1 week,2 weeks and 4 weeks after photocoagulation,the nature process and incidence of CNV were observed by light microscopy (LM). Results 2 hours and 3 days after laser treatment,there were no CNV and fluorescent leakage observed in rats.The first singns of CNV appeared at one week.Histological sections proved the excistence of CNV and the Incidence of CNV was 35.7%,72.2% and 70.6% at one week,two weeks and four weeks respectively. Conclusion The animal model is suitable for studying choroidal angiogenesis in vivo,especially in regard to the chemotherapeutic agents and gene targeting.