VEGF及其受体KDR在食管鳞状细胞癌组织中的表达及其临床意义

The Expression of VEGF and Its Recepter KDR among Esophageal Squamous Cell Carcinoma and Its Clinical Value

目的研究食管鳞状细胞癌组织中血管内皮生长因子 (vascularendothelialgrowthfactor ,VEGF)及其受体KDR(kinaseinsertdomaincotainingreceptor fetalliverkinase 1)的表达及与食管癌生物学行为的相关性。方法采用免疫组织化学技术 ,检测 5 0例手术切除、病理证实的食管鳞状细胞癌组织VEGF、KDR的蛋白表达。结果VEGF主要在肿瘤细胞表达 ,KDR在间质细胞、肿瘤细胞、腺体及血管内皮细胞均有表达 ,并与VEGF有相似性 ,癌巢边缘阳性表达降低。结论VEGF、KDR与食管鳞状细胞癌的一些生物学行为有一定的相关性。

Objective To investigate the correlation among the expression of vascular endothelial growth factor (VEGF),its receptor KDR(kinase insert domain cotaining receptor /fetal liver kinase-1) and the biological behaviors of esophageal squamous cell carcinoma. Methods Fifty cases of esophageal squamous cell carcinoma confirmed by surgery and pathology were studied with immunohistochemical technique to determine the protein expression of VEGF and its receptor KDR in esophageal squamous cell carcinoma tissue.The statistic methods were Spearman′s rank correlation and one-way analysis of variance(ANOVA) after data type conversion for normal distribution. Results It was discovered that the main source of the VEGF production was the tumor cells themselves.KDR was expressed in tumor cells and stroma, glands, vascular endothelial cells as well. The expression of both substances had decreased in the edge of tumor nest. The Spearman′s rank correlation between VEGF,KDR and clinico pathological staging were r_S=0.767,r_S=0.659 respectively(P<0.001 respectively).The correlation between VEGF,KDR and differentiation degrees of the tumor were r_s=-0.335and r_s=-0.271 respectively(P<0.05). The correlation between VEGF,KDR and lymphnode metastases were r_s=0.663 and r_s=0.572 respectively(P<0.001).The positive rates of VEGF,KDR in lymphnode-metastasis cases and non-metastasis cases were31.8%,25.4% and 11.7%,10.9% respectively.The differences were both significant(P<0.01). The expression of VEGF,KDR in esophageal cancer tissues had no relation to the location and lenth of the tumor.The expression of VEGF had no relation to clinical experiences while KDR had (P<0.05).The expression of VEGF had relation to age (P<0 05 respectively),which may explain why the elder patients had little incidence of lymphatic metastasis and better prognosis in clinical practice. Conclusion The results indicate that VEGF,KDR had relations to some biological behaviors of esophageal squamous cell carcinoma.The expression of VEGF and KDR had a positive correlation to clinico-pathological staging.VEGF and KDR may also indicate whether a lymphatic metastasis occurred.