高效液相色谱法同时测定双嘧达莫和阿司匹林的血药浓度

Establishment of a HPLC method for the determination of dipyridamole and aspirin in plasma

目的建立高效液相色谱法测定血浆中双嘧达莫及阿司匹林的浓度。方法 色谱柱:Hypersil BDS(200 mm×4.6 mm5μm,大连依利特);流动相:乙腈-甲醇-水-磷酸-二乙胺(50:250:200:0.3:0.1):流速:0.9 mL·min-1;检测波长:288 nm。结果阿司匹林、双嘧达莫在0.1-16μg·mL-1浓度内进样量与色谱峰面积呈良好的线性关系。阿司匹林r=0.994 2,双嘧达莫,r=0.994 7,回收率分别为101.21％,100.35％,精密度测定RSD小于1.78％。阿司匹林及双嘧达莫的tmax分别为1.75和3.33 h,cmax分别为7.71,3.68μg·mL-1,t1/2分别为2.71,3.13 h。结论该方法灵敏、准确、经济。用此法测定了家犬口服复方双嘧达莫缓释胶囊的血药浓度并计算了药动学参数。

OBJECTIVE: A HPLC method was established for the determination of dipyridamole and aspirin in plasma. METHODS: Analytical column was Spherisorb C18. The mobile phase consisted of acetonitrile-methol-water-H 3PO4-triethyamine(50:250:200:0.3:0.1). The flow rate was 0.9 mL&middotmin-1. The detection was performed at 288 nm. RESULTS: The calibration curves of aspirin and dipyridamole were linear in the concentration ranged from 0.1 to 16 μg&middotmL-1 (r = 0.994 2 and r = 0.994 7, respectively). The recoveries were 101.21% and 100.35%, respectively. RSD was less than 1.78%. The pharmacokinetic parameters of aspirin and dipyridamole in dogs were as follows: tmax, 1.75 and 3.33 h; cmax, 7.71 and 3.68 μg&middotmL-1; t1/2, 2.71 and 3.13 h; CONCLUSION: The method is accurate and can be used for studying the pharmacokinetics and bioavailability of aspirin and dipyridamole.