PAS、AB染色法在软骨蛋白黏多糖检测中的运用

The application of Periodic acid Schiff (PAS) and Alcian blue (AB) stains in proteoglycan detection of articular cartilage

[目的 ] 探讨过碘酸雪夫氏反应PAS染色 (PeriodicAcidSchiff)及阿利辛兰AB染色 (AlcianBlue)组织化学分析法在检测软骨组织蛋白黏多糖含量中的运用价值。 [方法 ] 以原发性退行性骨关节炎动物模型的软骨为检测标本 ,分别采用PAS和AB染色法 ,观察软骨组织中蛋白黏多糖的含量。 [结果 ] PAS染色法中软骨组织基质中的中性黏多糖呈紫红色 ,软骨细胞内为无色。AB法中软骨细胞周围的酸性黏多糖呈深蓝色 ,基质呈淡蓝色。此外 ,随着动物模型关节炎程度的不断加剧 ,即软骨组织中蛋白黏多糖丢失的不断增加 ,标本中的紫红色和深蓝色也相应减退 ,直至消失。 [结论 ] PAS染色法和AB染色法能清楚地区分软骨基质中的中性黏多糖和细胞囊内的酸性黏多糖 ,并能对它们的改变进行量化分析。可作为软骨组织中黏多糖检测的有效方法。

Objective] Feasibility study on histochemical methods of PAS and AB stains applied in proteoglycan examination in articular cartilage. [Methods] Proteoglycan in articular cartilage specimens from osteoarthritis animal models were measured with PAS and Alcain Blue stains respectively. [Results] Neutral proteoglycan in cell matrix showed purplish red by PAS stain, while acidic proteoglycan in chondrocyte periphery showed navy blue by AB stain. Along with the increasing of OA damage which the concentrations of proteoglycan in cartilage tissues were decreased, the colors of purplish red and navy blue in specimens had associated fading. [Conclusion] PAS stain and AB stain show proteoglycan existing in specimens, and differentiate proteoglycan between neutral and acidic. The concentrations of proteoglycan are quantified simultaneously. It indicate that PAS stain and AB stain are effective in proteoglycan detection in articular cartilage.